Biology:dnaC

From HandWiki

DNA replication gene dnaC
Identifiers
OrganismEscherichia coli
(str. K-12 substr. MG1655)
SymboldnaC
Entrez948864
RefSeq (Prot)NP_418781.1
UniProtP0AEF0
Other data
EC number2.7.7.7
Chromosomegenome: 4.6 - 4.6 Mb

dnaC is a loading factor that complexes with the C-terminus of helicase dnaB and inhibits it from unwinding the dsDNA at a replication fork.[1] A dnaB and dnaC associate near the dnaA bound origin for each of the ssDNA.[1] One dnaB-dnaC complex is oriented in the opposite direction to the other dnaB-dnaC complex due to the antiparallel nature of DNA. Because they are oriented in opposite directions, one dnaB-dnaC complex will complex with dnaA from the N-terminus of dnaB whereas the other dnaB-dnaC complex will complex with dnaA from the dnaC. After the assembly of dnaG onto the N-terminus of dnaB, dnaC is released and dnaB will be allowed to begin unwinding dsDNA to make room for DNA polymerase III to begin synthesizing the daughter strands.[1]

This interaction of dnaC with dnaB requires the hydrolysis of ATP.[2]

References

  1. 1.0 1.1 1.2 Slonczewski, Joan L.; Foster, John W.; Gillen, Kathy M. (2009). Microbiology: an evolving science (1st ed.). New York: W.W. Norton & Co.. ISBN 978-0-393-97857-5. 
  2. "The Escherichia coli dnaC gene product. III. Properties of the dnaB-dnaC protein complex". The Journal of Biological Chemistry 257 (22): 13770–5. November 1982. PMID 6292205. http://www.jbc.org/cgi/content/abstract/257/22/13770. 

External links