Biology:2-hydroxy-dATP diphosphatase

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Short description: Class of enzymes
2-hydroxy-dATP diphosphatase
Identifiers
EC number3.6.1.56
Databases
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BRENDABRENDA entry
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KEGGKEGG entry
MetaCycmetabolic pathway
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2-hydroxy-dATP diphosphatase (EC 3.6.1.56, also known as oxidized purine nucleoside triphosphatase, or (2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase, or Nudix hydrolase 1 (NUDT1), or MutT homolog 1 (MTH1), or 7,8-dihydro-8-oxoguanine triphosphatase) is an enzyme that in humans is encoded by the NUDT1 gene.[1][2][3] During DNA repair, the enzyme hydrolyses oxidized purines and prevents their addition onto the DNA chain. As such it has important role in aging and cancer development.

Function

This enzyme catalyses the following chemical reaction

2-hydroxy-dATP + H2O [math]\displaystyle{ \rightleftharpoons }[/math] 2-hydroxy-dAMP + diphosphate

The enzyme hydrolyses oxidized purine nucleoside triphosphates. The enzyme is used in DNA repair, where it hydrolysis the oxidized purines and prevents their addition onto the DNA chain.[4]

Misincorporation of oxidized nucleoside triphosphates into DNA and/or RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. First isolated from Escherichia coli because of its ability to prevent occurrence of 8-oxoguanine in DNA,[5] the protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation.File:MTH1 Hydrolysis of Oxidized Nucleotide.tif MutT enzymes in non-human organisms often have substrate specificity for certain types of oxidized nucleotides, such as that of E. coli, which is specific to 8-oxoguanine nucleotides. Human MTH1, however, has substrate specificity for a much broader range of oxidatively damaged nucleotides. The mechanism of hMTH1's broad specificity for these oxidized nucleotides is derived from their recognition in the enzyme's substrate binding pocket due to an exchange of protonation state between two nearby aspartate residues.[6]File:MTH1 Recognition of Oxidized Nucleotides.tif The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. In plants, MTH1 has also been shown to enhance resistance to heat- and paraquat-induced oxidative stress, resulting in fewer dead cells and less accumulation of hydrogen peroxide.[7]

Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform has been described.[3]

Research

Aging

A mouse model has been studied that over-expresses hMTH1-Tg (NUDT1).[8] The hMTH1-Tg mice express high levels of the hMTH1 hydrolase that degrades 8-oxodGTP and 8-oxoGTP and therefore excludes 8-oxoguanine from DNA and RNA. The steady state levels of 8-oxoguanine in DNA of several organs including the brain are significantly reduced in hMTH1-Tg over-expressing mice. Conversely, MTH1-null mice exhibit a significantly higher level of 8-oxo-dGTP accumulation than that of the wild type.[9] Over-expression of hMTH1 prevents the age-dependent accumulation of DNA 8-oxoguanine that occurs in wild-type mice. The lower levels of oxidized guanines are associated with greater longevity. The hMTH1-Tg animals have a significantly longer lifespan than their wild-type littermates. These findings provide a link between ageing and oxidative DNA damage[8] (see DNA damage theory of aging).

Cancer

Studies have suggested that this enzyme plays a role in both preventing the formation of cancer cells and the proliferation of cancer cells. This makes it a topic of interest in cancer research, both as a potential method for healthy cells to prevent cancer and a weakness to target within existing cancer cells.

Eliminating the MTH1 gene in mice results in over three times more mice developing tumors compared to a control group.[10] The enzyme's much-studied ability to sanitize a cell's nucleotide pool prevents it from developing mutations, including cancerous ones. Specifically, another study found that MTH1 inhibition in cancer cells leads to incorporation of 8-oxo-dGTP and other oxidatively damaged nucleotides into the cell's DNA, damaging it and causing cell death.[11] However, cancer cells have also been shown to benefit from use of MTH1. Cells from malignant breast tumors exhibit extreme MTH1 expression compared to other human cells.[12] Because a cancer cell divides much more rapidly than a normal human cell, it is far more in need of an enzyme like MTH1 that prevents fatal mutations during replication. This property of cancer cells could allow for monitoring of cancer treatment efficacy by measuring MTH1 expression. Development of suitable probes for this purpose is currently underway.[13][14]

Disagreement exists concerning MTH1's functionality relative to prevention of DNA damage and cancer. Subsequent studies have had difficulty reproducing previously reported cytotoxic or antiproliferation effects of MTH1 inhibition on cancer cells, even calling into question whether MTH1 truly does serve to remove oxidatively damaged nucleotides from a cell's nucleotide pool.[15][16] One study of newly discovered MTH1 inhibitors suggests that these anticancer properties exhibited by the older MTH1 inhibitors may be due to off-target cytotoxic effects.[17] After revisiting the experiment, the original authors of this claim found that while the original MTH1 inhibitors in question lead to damaged nucleotides being incorporated into DNA, they demonstrate the others that do not induce toxicity fail to introduce the DNA lesion.[18] Research into this topic is ongoing.

As a drug target

MTH1 is a potential drug target to treat cancer, however there are conflicting results regarding the cytotoxicity of MTH1 inhibitors toward cancer cells.[19]

Karonudib, an MTH1 inhibitor, is currently being evaluated a phase I clinical trial for safety and tolerability.[18][20][21]

A potent and selective MTH1 inhibitor AZ13792138 has been developed by AstraZeneca has been made available as a chemical probe to academic researchers.[22] However AstraZeneca has found that neither AZ13792138 nor genetic knockdown of MTH1 displays any significant cytotoxicity to cancer cells.[23][24]

See also

References

  1. ↑ "Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB)". Genomics 24 (3): 440–4. December 1994. doi:10.1006/geno.1994.1650. PMID 7713494. 
  2. ↑ "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis". The Journal of Biological Chemistry 268 (31): 23524–30. November 1993. doi:10.1016/S0021-9258(19)49494-5. PMID 8226881. 
  3. ↑ 3.0 3.1 "Entrez Gene: NUDT1 nudix (nucleoside diphosphate linked moiety X)-type motif 1". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4521. 
  4. ↑ Rudling, Axel; Gustafsson, Robert; Almlöf, Ingrid; Homan, Evert; Scobie, Martin; Warpman Berglund, Ulrika; Helleday, Thomas; Stenmark, Pål et al. (2017-10-12). "Fragment-Based Discovery and Optimization of Enzyme Inhibitors by Docking of Commercial Chemical Space". Journal of Medicinal Chemistry 60 (19): 8160–8169. doi:10.1021/acs.jmedchem.7b01006. ISSN 1520-4804. PMID 28929756. 
  5. ↑ "8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity". Proceedings of the National Academy of Sciences of the United States of America 88 (11): 4690–4. June 1991. doi:10.1073/pnas.88.11.4690. PMID 2052552. Bibcode: 1991PNAS...88.4690T. 
  6. ↑ 6.0 6.1 "Structural and Kinetic Studies of the Human Nudix Hydrolase MTH1 Reveal the Mechanism for Its Broad Substrate Specificity". The Journal of Biological Chemistry 292 (7): 2785–2794. February 2017. doi:10.1074/jbc.m116.749713. PMID 28035004. 
  7. ↑ "Ectopic expression of the human MutT-type Nudix hydrolase, hMTH1, confers enhanced tolerance to oxidative stress in arabidopsis". Plant & Cell Physiology 55 (9): 1534–43. September 2014. doi:10.1093/pcp/pcu083. PMID 24928220. 
  8. ↑ 8.0 8.1 "Prolonged lifespan with enhanced exploratory behavior in mice overexpressing the oxidized nucleoside triphosphatase hMTH1". Aging Cell 12 (4): 695–705. August 2013. doi:10.1111/acel.12094. PMID 23648059. 
  9. ↑ "MTH1, an oxidized purine nucleoside triphosphatase, suppresses the accumulation of oxidative damage of nucleic acids in the hippocampal microglia during kainate-induced excitotoxicity". The Journal of Neuroscience 26 (6): 1688–98. February 2006. doi:10.1523/jneurosci.4948-05.2006. PMID 16467516. 
  10. ↑ "Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase". Proceedings of the National Academy of Sciences of the United States of America 98 (20): 11456–61. September 2001. doi:10.1073/pnas.191086798. PMID 11572992. Bibcode: 2001PNAS...9811456T. 
  11. ↑ "MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool". Nature 508 (7495): 215–21. April 2014. doi:10.1038/nature13181. PMID 24695224. Bibcode: 2014Natur.508..215G. 
  12. ↑ "Addiction to MTH1 protein results in intense expression in human breast cancer tissue as measured by liquid chromatography-isotope-dilution tandem mass spectrometry". DNA Repair 33: 101–10. September 2015. doi:10.1016/j.dnarep.2015.05.008. PMID 26202347. 
  13. ↑ "A Chimeric ATP-Linked Nucleotide Enables Luminescence Signaling of Damage Surveillance by MTH1, a Cancer Target". Journal of the American Chemical Society 138 (29): 9005–8. July 2016. doi:10.1021/jacs.6b02895. PMID 27413803. 
  14. ↑ Samaranayake, Govindi J.; Troccoli, Clara I.; Huynh, Mai Q.; Win, Andrew; Ji, Debin; Kool, Eric T.; Rai, Priyamvada (July 2017). "Towards a better understanding of MTH1 as a therapeutic target in RAS-driven cancer [abstract]". Cancer Res. 77 (13): 5473. doi:10.1158/1538-7445.AM2017-5473. 
  15. ↑ "Identification of potent and selective MTH1 inhibitors". Bioorganic & Medicinal Chemistry Letters 26 (6): 1503–7. March 2016. doi:10.1016/j.bmcl.2016.02.026. PMID 26898335. 
  16. ↑ "Novel Class of Potent and Cellularly Active Inhibitors Devalidates MTH1 as Broad-Spectrum Cancer Target". ACS Chemical Biology 12 (8): 1986–1992. July 2017. doi:10.1021/acschembio.7b00370. PMID 28679043. 
  17. ↑ "Potent and Selective Inhibitors of MTH1 Probe Its Role in Cancer Cell Survival". Journal of Medicinal Chemistry 59 (6): 2346–61. March 2016. doi:10.1021/acs.jmedchem.5b01760. PMID 26878898. 
  18. ↑ 18.0 18.1 "Validation and development of MTH1 inhibitors for treatment of cancer". Annals of Oncology 27 (12): 2275–2283. December 2016. doi:10.1093/annonc/mdw429. PMID 27827301. 
  19. ↑ "MTH1 as a Chemotherapeutic Target: The Elephant in the Room". Cancers 9 (5): 47. 2017. doi:10.3390/cancers9050047. PMID 28481306. 
  20. ↑ Thomas, Ashley (21 September 2016). "The Helleday Team presents potent MTH1 inhibitor 'Karonudib' and experimentally addresses the non-active MTH1 inhibitors.". http://helleday.org/the-helleday-team-presents-potent-mth1-inhibitor-karonudib-and-experimentally-addresses-the-non-active-mth1-inhibitors-and-described-mth1-clones/. 
  21. ↑ "MTH1, A Phase I, Study on Tumors Inhibition, First in Human, First in Class (MASTIFF)". U.S. National Institutes of Health. 14 February 2017. https://clinicaltrials.gov/ct2/show/NCT03036228. 
  22. ↑ "AZ13792138". AstraZeneca. 2016. https://openinnovation.astrazeneca.com/az13792138.html. 
  23. ↑ "Potent and Selective Inhibitors of MTH1 Probe Its Role in Cancer Cell Survival". Journal of Medicinal Chemistry 59 (6): 2346–61. 2016. doi:10.1021/acs.jmedchem.5b01760. PMID 26878898. 
  24. ↑ "MutT Homolog 1 (MTH1): The Silencing of a Target". Journal of Medicinal Chemistry 59 (6): 2343–5. 2016. doi:10.1021/acs.jmedchem.6b00283. PMID 26924380. 

Further reading

External links



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