Chemistry:Seminaphtharhodafluor
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| Other names
Seminaphtharhodafluorescein; Seminaphthorhodafluor
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3D model (JSmol)
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PubChem CID
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| Properties | |
| C24H15NO4 | |
| Molar mass | 381.387 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Seminaphtharhodafluor or SNARF is a fluorescent dye that changes color with pH. It can be used to construct optical biosensors that use enzymes that change pH.
The absorption peak of the derivative carboxy-SNARF[specify] at pH 6.0 is at wavelength (515 and) 550 nm, while that at pH 9.0 is at 575 nm.[citation needed]
The emission peak of carboxy-SNARF at pH 6.0 is at wavelength 585 nm, while that at pH 9.0 is at 640 nm.[1]
SNARF-1 can serve as a substrate for the MRP1 (multidrug resistance-associated protein-1) drug transporter, to measure the activity of the MRP1 transporter. For this purpose, an acetomethoxyester group is added to SNARF-1. Cellular esterases cleave off SNARF-1, and its transport out of the cells can be measured by following the loss of fluorescence from the cells.[2]
References
- ↑ "5-(and-6)-Carboxy SNARF™-1". http://www.lifetechnologies.com/order/catalog/product/C1270.
- ↑ Weekes, M. P.; Tan, S. Y. L.; Poole, E.; Talbot, S.; Antrobus, R.; Smith, D. L.; Montag, C.; Gygi, S. P. et al. (12 April 2013). "Latency-Associated Degradation of the MRP1 Drug Transporter During Latent Human Cytomegalovirus Infection". Science 340 (6129): 199–202. doi:10.1126/science.1235047. PMID 23580527. Bibcode: 2013Sci...340..199W.
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