Chemistry:Estrogen provocation test
| Estrogen provocation test | |
|---|---|
| Medical diagnostics | |
| Synonyms | Estrogen stimulation test; Estrogen challenge test |
| Purpose | Evaluate hypothalamic–pituitary–gonadal function |
The estrogen provocation test, also known as the estrogen stimulation test or estrogen challenge test, is a diagnostic procedure used to evaluate the function of the hypothalamic–pituitary–gonadal axis.[2][3][4] It involves the administration of a large amount of estrogen, resulting in estrogenic exposure similar to or greater than normal preovulatory estradiol levels, in an attempt to induce a positive feedback surge in levels of the gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH).[2][3] Estrogens that have been used in the estrogen provocation test include estradiol benzoate, estradiol valerate, ethinylestradiol, and high-dose transdermal estradiol patches.[2][3][4][5] The test involves sustained estrogenic exposure equivalent to estradiol levels of 200 to 300 pg/mL or more for at least 50 hours[6] and results in a surge in gonadotropin levels about 32 to 72 hours following initiation of estrogenic exposure.[7][5] Levels of LH and FSH increase during the gonadotropin surge by about 10-fold and 4-fold, respectively.[5]
See also
- Progestogen challenge test
- Pharmacodynamics of estradiol § Progonadotropic effects
References
- ↑ Shaw, Robert W. (November 1978). "Neuroendocrinology of the menstrual cycle in humans". Clinics in Endocrinology and Metabolism 7 (3): 531–559. doi:10.1016/S0300-595X(78)80008-5. ISSN 0300-595X. PMID 365398.
- ↑ 2.0 2.1 2.2 "Tests of the hypothalamic-pituitary-ovarian axis". Clinics in Obstetrics and Gynaecology 3 (3): 485–503. December 1976. doi:10.1016/S0306-3356(21)00360-5. PMID 1009729.
- ↑ 3.0 3.1 3.2 "The LH surge in humans: its mechanism and sex difference". Gynecol Endocrinol 2 (2): 165–82. June 1988. doi:10.3109/09513598809023624. PMID 3055821.
- ↑ 4.0 4.1 "The oestrogen provocation test: a method of assessing the hypothalamic-pituitary axis in patients with amenorrhoea". Clinical Endocrinology 4 (3): 267–76. May 1975. doi:10.1111/j.1365-2265.1975.tb01534.x. PMID 1149302.
- ↑ 5.0 5.1 5.2 McCartney, Christopher R; Gilrain, Melissa; Lundgren, Jessica A; Kim, Su Hee; Solorzano, Christine Michele Burt (2021-05-01). "Assessment of Estradiol-Induced Gonadotropin Surge Generation: Preliminary Results From a Protocol of Graded Transdermal Estradiol Dosing". Journal of the Endocrine Society (The Endocrine Society) 5 (Supplement_1): A729–A730. doi:10.1210/jendso/bvab048.1484. ISSN 2472-1972.
- ↑ "Strength-duration characteristics of estrogen effects on gonadotropin response to gonadotropin-releasing hormone in women. II. Effects of varying concentrations of estradiol". J Clin Endocrinol Metab 42 (3): 432–42. March 1976. doi:10.1210/jcem-42-3-432. PMID 767352.
- ↑ "Neuroendocrinology of the menstrual cycle in humans". Clin Endocrinol Metab 7 (3): 531–59. November 1978. doi:10.1016/s0300-595x(78)80008-5. PMID 365398.
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