Biology:Sf9 (cells)
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Short description: Insect cell line
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue.[1] They can be grown in the absence of serum, and can be cultured attached or in suspension.[2]
Sf9 Rhabdovirus
It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV).[3][4] However, not all tested Sf9 cells appear to be infected with this virus.[5] SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.[6]
References
- ↑ Vaughn JL; Goodwin RH; Tompkins GJ; McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913.
- ↑ Invitrogen (14 Mar 2017). Growth and Maintenance of Insect cell lines: User Guide. Invitrogen. Manual part no. 25-0127, MAN0000030. https://tools.thermofisher.com/content/sfs/manuals/Insect_Cell_Lines_UG.pdf. Retrieved 12 May 2017.
- ↑ Ma H, Galvin TA, Glasner DR, Shaheduzzaman S, Khan AS (2014). "Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.". J Virol 88 (12): 6576–85. doi:10.1128/JVI.00780-14. PMID 24672045.
- ↑ Parry R, de Malmanche H, Asgari S (2021). "Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery.". Virology 563: 82–87. doi:10.1016/j.virol.2021.08.013. PMID 34492433.
- ↑ Hashimoto Y, Macri D, Srivastava I, McPherson C, Felberbaum R, Post P (2017). "Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line.". PLOS ONE 12 (4): e0175633. doi:10.1371/journal.pone.0175633. PMID 28423032. Bibcode: 2017PLoSO..1275633H.
- ↑ Maghodia AB, Jarvis DL (2017). "Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines.". Virology 512: 234–245. doi:10.1016/j.virol.2017.09.025. PMID 29024851.
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