Biology:Rhizorhabdus wittichii
| Rhizorhabdus wittichii | |
|---|---|
| |
Scientific classification 
| |
| Domain: | Bacteria |
| Phylum: | Pseudomonadota |
| Class: | Alphaproteobacteria |
| Order: | Sphingomonadales |
| Family: | Sphingomonadaceae |
| Genus: | Rhizorhabdus |
| Species: | R. wittichii
|
| Binomial name | |
| Rhizorhabdus wittichii (Yabuuchi et al. 2001) Hördt et al. 2020[1]
| |
| Synonyms | |
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Sphingomonas wittichii Yabuuchi et al. 2001 | |
The species Rhizorhabdus wittichii, formerly Sphingomonas wittichii, is a Gram-negative, rod-shaped motile bacterium, with an optimum growth temperature at 30 °C.[2] It forms a greyish white colony.[3] It has been found to have a 67 mol% of DNA G+C content.[3]
The R. wittichii RW1 genome consists of 5,915,246 bp and consists of a single circular chromosome and two plasmids.[4]
Background
It was first isolated from water of the River Elbe by R.-M. Wittich, after whom the species is named.[2]
The species was originally thought to belong to the genus Sphingomonas, despite poor alignment of its 16S rRNA gene with its putative nearest neighbor.[5] It has since been reclassified to Rhizorhabdus as part of a larger re-evaluation of Alphaproteobacteria.[6]
Strain(s)
Its type strain is R. wittichii RW1 DSM 6014T (= JCM 10273T = EY 4224T).[7]
Mechanism and biotechnological applications
R. wittichii RW1 is notable for metabolising dibenzo-p-dioxin and phenazine-1-carboxylic acid.[8] In fact, Sphingomonas wittichii strain RW1 (RW1) is one of the very few strains that can grow on dibenzo-p-dioxin (DD).[9] Furthermore, this bacterium also grows on dibenzofuran and 4-chloro-dibenzofuran, using them as the sole carbon sources.[9] Such biodegradative capabilities are not unique to this strain.
R. wittichii MPO218 degrades ibuprofen, carrying degradative genes on a large plasmid.[10] Thanks to its wide-ranging metabolic capabilities and likely propensity to acquire novel degradation genes, in no small part due to its wealth of plasmids,[11]
The unusual arrangement of its genes involved in dioxin degradation, and the full description of the dioxin degradation pathway, is still under investigation.
This organism holds a high potential for biotechnological applications.[5]
References
- ↑ Page Species: Rhizorhabdus wittichii on "LPSN - List of Prokaryotic names with Standing in Nomenclature". Deutsche Sammlung von Mikroorganismen und Zellkulturen. https://lpsn.dsmz.de/species/rhizorhabdus-wittichii.
- ↑ 2.0 2.1 "Proposal of Sphingomonas wittichii sp. nov. for strain RW1T, known as a dibenzo-p-dioxin metabolizer". International Journal of Systematic and Evolutionary Microbiology 51 (Pt 2): 281–292. March 2001. doi:10.1099/00207713-51-2-281. PMID 11321072.
- ↑ 3.0 3.1 "Sphingoaurantiacus polygranulatus gen. nov., sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii". International Journal of Systematic and Evolutionary Microbiology 66 (1): 91–100. January 2016. doi:10.1099/ijsem.0.000677. PMID 26475309.
- ↑ "PATRIC". https://patricbrc.org/view/Genome/392499.14.
- ↑ 5.0 5.1 "Home - Sphingomonas wittichii RW1". https://genome.jgi.doe.gov/portal/sphwi/sphwi.home.html.
- ↑ "Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of 'Alphaproteobacteria'". Frontiers in Microbiology 11: 468. 2020. doi:10.3389/fmicb.2020.00468. PMID 32373076.
- ↑ "Rhizorhabdus wittichii RW1 | Type strain | DSM 6014, JCM 10273, JCM 15750, CCUG 31198, CIP 107293, KCTC 12908, MTCC 7933, NBRC 100425, NBRC 105917 | BacDiveID:14228" (in en). https://bacdive.dsmz.de/strain/14228.
- ↑ "Genome sequence of Sphingomonas wittichii DP58, the first reported phenazine-1-carboxylic acid-degrading strain". Journal of Bacteriology 194 (13): 3535–3536. July 2012. doi:10.1128/JB.00330-12. PMID 22689229.
- ↑ 9.0 9.1 "Sphingomonas wittichii Strain RW1 Genome-Wide Gene Expression Shifts in Response to Dioxins and Clay". PLOS ONE 11 (6): e0157008. 2016-06-16. doi:10.1371/journal.pone.0157008. PMID 27309357. Bibcode: 2016PLoSO..1157008C.
- ↑ "Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218". Applied and Environmental Microbiology 88 (11): e0038822. June 2022. doi:10.1128/aem.00388-22. PMID 35604231. Bibcode: 2022ApEnM..88E.388A.
- ↑ "Detection and characterization of conjugative degradative plasmids in xenobiotic-degrading Sphingomonas strains". Journal of Bacteriology 186 (12): 3862–3872. June 2004. doi:10.1128/JB.186.12.3862-3872.2004. PMID 15175300.
Further reading
- "Genome sequence of the dioxin-mineralizing bacterium Sphingomonas wittichii RW1". Journal of Bacteriology 192 (22): 6101–6102. November 2010. doi:10.1128/JB.01030-10. PMID 20833805.
- "The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein". Biopolymers 93 (9): 811–822. September 2010. doi:10.1002/bip.21482. PMID 20578000. https://www.research.ed.ac.uk/en/publications/56f0afdf-c010-42c7-826c-c92d06fcee39.
External links
Wikidata ☰ Q112996810 entry
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