Biology:H295R
H295R (also referred to as NCI-H295R) is an angiotensin-II-responsive steroid-producing adrenocortical cell line.[1] It was initially isolated in 1980 from a 48-year-old female patient diagnosed with adrenocortical carcinoma.[1][2] The initial polyclonal populations of tumor cells obtained from the patients' tumor were cultured and the resultant cell line was called NCI-H295.[1][2] Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth.[1] Three strains were developed, based on the serum supplement used for growth, which have been termed H295R-S1, H295R-S2 and H295R-S3.[1][3] All three strains grow as adherent monolayer cultures.[1]
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 "Human Adrenocortical Carcinoma Cell Lines.". Mol. Cell. Endocrinol. 351 (1): 58–65. 2012. doi:10.1016/j.mce.2011.08.041. PMID 21924324.
- ↑ 2.0 2.1 "Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis.". Cancer Res. 50 (17): 5488–5496. 1990. PMID 2386954. http://cancerres.aacrjournals.org/content/50/17/5488.long.
- ↑ "Adrenocortical cell lines.". Mol. Cell. Endocrinol. 228 (1–2): 23–38. 2004. doi:10.1016/j.mce.2003.12.020. PMID 15541570.
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