Biology:Baird-Parker agar

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Short description: Selective culture medium
Staphylococcus growth in Baird-Parker (BP) agar.

Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species.[1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci.[1] Staphylococcus colonies show up black in colour with clear zones produced around them.[1]

History

Baird-Parker first published an academic article about this agar medium for the purposes of improved diagnostics and isolating coagulase-positive Staphylococci in 1962.[2] He developed this agar medium from the tellurite-glycine formulation of Zebovitz et al[3] and improved its reliability in isolating coagulase-positive staphylococci from foods. Baird-Parker added egg yolk emulsion as a diagnostic agent and sodium pyruvate to protect damaged cells and aid their recovery.[4] It is now widely recommended by national and international bodies for the isolation of coagulase-positive staphylococci.[5] Braid-Parker agar is commonly used as a method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) in food and animal feedstuffs.[6]

References

  1. ↑ 1.0 1.1 1.2 "Baird-Parker Agar Base". EMD Chemicals. Archived from the original on 2008-05-01. https://web.archive.org/web/20080501041929/http://www.emdchemicals.com/analytics/Micro_Manual/TEDISdata/prods/1_05406_0500.html. Retrieved 2008-05-16. 
  2. ↑ Baird-Parker, A. C. (1962). "An Improved Diagnostic and Selective Medium for Isolating Coagulase Positive Staphylococci" (in en). Journal of Applied Bacteriology 25 (1): 12–19. doi:10.1111/j.1365-2672.1962.tb01113.x. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1962.tb01113.x. 
  3. ↑ Zebovitz, Eugene; Evans, James B.; Niven, C. F. (1955). "Tellurite-Glycine Agar: A Selective Plating Medium for the Quantitative Detection of Coagulase-Positive Staphylococci". Journal of Bacteriology 70 (6): 686–690. doi:10.1128/jb.70.6.686-690.1955. ISSN 0021-9193. PMID 13271314. PMC 386272. http://dx.doi.org/10.1128/jb.70.6.686-690.1955. 
  4. ↑ Baird-Parker, A. C.YR 1963 (1963). "A Classification of Micrococci and Staphylococci Based on Physiological and Biochemical Tests". Microbiology 30 (3): 409–427. doi:10.1099/00221287-30-3-409. ISSN 1465-2080. PMID 13969076. 
  5. ↑ CHOPIN, A.; MALCOLM, S.; JARVIS, G.; ASPERGER, H.; BECKERS, H. J.; BERTONA, A. M.; COMINAZZINI, C.; CARINI, S. et al. (1985-01-01). "ICMSF Methods Studies. XV. Comparison of Four Media and Methods for Enumerating Staphylococcus aureus in Powdered Milk". Journal of Food Protection 48 (1): 21–27. doi:10.4315/0362-028X-48.1.21. ISSN 0362-028X. PMID 30934501. 
  6. ↑ "Dehydrated Culture Media: BAIRD-PARKER AGAR (ISO) BASE". http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM1127&c=UK&lang=EN. 




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