Biology:Anaeromyces robustus

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Short description: Fungus living in the gut of cows and sheep

Anaeromyces robustus
Anaeromyces robustus 1.jpg
Scientific classification edit
Domain: Eukaryota
Kingdom: Fungi
Division: Neocallimastigomycota
Class: Neocallimastigomycetes
Order: Neocallimastigales
Family: Neocallimastigaceae
Genus: Anaeromyces
Species:
A. robustus
Binomial name
Anaeromyces robustus
O'Malley, Theodorou & Henske (2016)

Anaeromyces robustus is a fungal microorganism that lives in the gut rumen of many ruminant herbivores such as cows and sheep.[1] Previously thought to be protozoa from their flagellated zoospores, they are biomass degraders and help the animal by breaking down carbohydrates and plant materials from the food the animal ingests. This fungus, therefore, is anaerobic and lives without oxygen. Gut fungi are dramatically outnumbered by other organisms in the microbiome; however, they are important members of the gut microbiome in ruminants and hind-gut fermenters and play a key role in digestion.[2]

Taxonomy

Initially, this fungal species was isolated from sheep fecal material in the Santa Barbara Zoo in 2017.[3] With how this was found, it fit into the order of the Neocallimastigomycota as all species within this order live in the gut rumen of large herbivores.

Description

A. robustus is characterized by its long hyphal segments and its ability to reproduce asexually from flagellated zoospores. With the production of zoospores, this fungus's lifecycle is very representative of species of fungi from the phylum Chitridiomycota.[4] This fungus has polycentric sporangia development as many sporangia develop throughout the thallus. With this, there is also nucleus movement through many repeated divisions through the rhizoids.[5]

A. robustus grown in culture media (Bowen, Benjamin P., etal)

Ecology

As stated beforehand, this fungus helps animals with digestion of plant materials. Certain enzymes produced from A. robustus have significantly more enzymatic activity when compared to bacteria and other organisms inside of the gut rumen. Other gut bacteria and archaea, however, can have a positive impact on the way A. robustus functions. There is a species of archaea, Methanobacterium bryantii, that enhances the function of the fungus.[6] In particular, this archaea increases the fungus's ability to break down carbohydrates by increasing the CAZyme production of the fungus. When exposed to harmful gut bacteria, rumen fungi are known to produce their own antibiotics, making them potentially useful when looking into synthesizing new antibiotics.[7]

Horizontal gene transfer (HGT) has been thought to have occurred between fungal ruminants, like A. robustus, and bacteria that are found in the same environment. Through this gene transfer, it is thought that it has adopted some of the biosynthetic genes the fungus uses to break down carbohydrates from certain bacteria.[8]

Anaerobic gut fungi can create a compound called styrylprone that is typically found in other mushrooms that can be used for herbal medicine. This compound has similarities to antioxidants that are found in a plethora of plants.[9]

Geographical distribution

Found in the United States around the country in many large ruminants and hind-gut fermenters. One reference states A. robustus and other ruminomyces were found in fecal samples from many cows and goats in Oklahoma.[10] Another reference states the fungus has been found in fecal samples of sheep in the Santa Barbara Zoo.[11]

Role in the Rumen

Although the rumen microbiome has been largely studied, the dramatic diversity of this fermentation site still leaves plenty of room for exploration. Ruminant animals such as cattle, sheep, and goats have a specialized four chamber stomach, the largest chamber of which is known as the rumen.[12] This rumen is home to a plethora of microorganisms that are responsible for the breakdown of fibrous materials.[12] This symbiotic relationship allows ruminants to utilize nutrients from plants which other species are unable to digest.[12]

Anaeromyces robustus is found in relatively low quantities compared to many bacterial microorganisms within the rumen; however, this fungus has been shown to play a significant role in ruminant digestion through the production of xylanase.[13] A large proportion of plants have a compound known as xylan within the hemicellulose in their cell walls. This compound can make it difficult for animals to utilize the nutrients within the cell. The xylanase, produced by Anaeromyces robustus,[13] is able to efficiently break down the xylan within plant cell walls, allowing ruminant animals to utilize the nutrients in fibrous feedstuffs. Although this fungus is well-established as a member of the ruminant digestive tract, fairly little is known about its genomics or the secondary metabolites it is responsible for producing.[2] Some sources have hypothesized that this fungi produces metabolites within the rumen which help the microorganism to survive in an environment where it is largely outnumbered by bacteria.[2]

Use in Bread Making

Recent studies have showed promising possible implications for Anaeromyces robustus as a contributor to bread-making.[13] Specifically, the gene within the fungus that produces xylanase has been identified in the lab for potential use in food production.[13] The introduction of this gene into flour used in baking has been shown to improve the appeal of bread by making the it softer and more palatable.[13] The fermentation temperatures required by the fungus are shown to closely match those required by yeast, another common component in bread, which could also further solidify its use in food production.[13]

References

  1. ↑ "Home - Anaeromyces robustus v1.0". https://mycocosm.jgi.doe.gov/Anasp1/Anasp1.home.html#:~:text=renewable%20biotechnology%20applications.-,Anaeromyces%20sp.,at%20the%20Santa%20Barbara%20Zoo.. 
  2. ↑ 2.0 2.1 2.2 Swift, Candice L.; Louie, Katherine B.; Bowen, Benjamin P.; Olson, Heather M.; Purvine, Samuel O.; Salamov, Asaf; Mondo, Stephen J.; Solomon, Kevin V. et al. (2021-05-04). "Anaerobic gut fungi are an untapped reservoir of natural products" (in en). Proceedings of the National Academy of Sciences 118 (18): e2019855118. doi:10.1073/pnas.2019855118. ISSN 0027-8424. PMID 33906945. Bibcode: 2021PNAS..11819855S. 
  3. ↑ Haitjema, Charles H.; Gilmore, Sean P.; Henske, John K.; Solomon, Kevin V.; de Groot, Randall; Kuo, Alan; Mondo, Stephen J.; Salamov, Asaf A. et al. (2017-05-30). "A parts list for fungal cellulosomes revealed by comparative genomics" (in en). Nature Microbiology 2 (8): 17087. doi:10.1038/nmicrobiol.2017.87. ISSN 2058-5276. PMID 28555641. https://www.nature.com/articles/nmicrobiol201787. 
  4. ↑ "Home - Anaeromyces robustus v1.0". https://mycocosm.jgi.doe.gov/Anasp1/Anasp1.home.html#:~:text=renewable%20biotechnology%20applications.-,Anaeromyces%20sp.,at%20the%20Santa%20Barbara%20Zoo.. 
  5. ↑ Chang, Jongsoo; Park, Hyunjin (2020). "Nucleotide and protein researches on anaerobic fungi during four decades". Journal of Animal Science and Technology 62 (2): 121–140. doi:10.5187/jast.2020.62.2.121. ISSN 2055-0391. PMID 32292921. PMC 7142291. http://www.ejast.org/archive/view_article?pid=jast-62-2-121. 
  6. ↑ Swift, Candice L; Brown, Jennifer L; Seppälä, Susanna; O’Malley, Michelle A (2019-10-01). "Co-cultivation of the anaerobic fungus Anaeromyces robustus with Methanobacterium bryantii enhances transcription of carbohydrate active enzymes" (in en). Journal of Industrial Microbiology and Biotechnology 46 (9–10): 1427–1433. doi:10.1007/s10295-019-02188-0. ISSN 1476-5535. PMID 31089985. 
  7. ↑ Swift, Candice L.; Louie, Katherine B.; Bowen, Benjamin P.; Hooker, Casey A.; Solomon, Kevin V.; Singan, Vasanth; Daum, Chris; Pennacchio, Christa P. et al. (2021-08-31). Taylor, John W.. ed. "Cocultivation of Anaerobic Fungi with Rumen Bacteria Establishes an Antagonistic Relationship" (in en). mBio 12 (4): e01442–21. doi:10.1128/mBio.01442-21. ISSN 2150-7511. PMID 34399620. 
  8. ↑ Swift, Candice L.; Louie, Katherine B.; Bowen, Benjamin P.; Olson, Heather M.; Purvine, Samuel O.; Salamov, Asaf; Mondo, Stephen J.; Solomon, Kevin V. et al. (2021-04-27). "Anaerobic gut fungi are an untapped reservoir of natural products" (in en-US). Proceedings of the National Academy of Sciences 118 (18). doi:10.1073/pnas.2019855118. ISSN 0027-8424. PMID 33906945. Bibcode: 2021PNAS..11819855S. 
  9. ↑ Swift, Candice L.; Louie, Katherine B.; Bowen, Benjamin P.; Olson, Heather M.; Purvine, Samuel O.; Salamov, Asaf; Mondo, Stephen J.; Solomon, Kevin V. et al. (2021-04-27). "Anaerobic gut fungi are an untapped reservoir of natural products" (in en-US). Proceedings of the National Academy of Sciences 118 (18). doi:10.1073/pnas.2019855118. ISSN 0027-8424. PMID 33906945. Bibcode: 2021PNAS..11819855S. 
  10. ↑ Hanafy, Radwa A.; Johnson, Britny; Elshahed, Mostafa S.; Youssef, Noha H. (2018-05-04). "Anaeromyces contortus, sp. nov., a new anaerobic gut fungal species (Neocallimastigomycota) isolated from the feces of cow and goat". Mycologia 110 (3): 502–512. doi:10.1080/00275514.2018.1465773. ISSN 0027-5514. PMID 29944464. https://doi.org/10.1080/00275514.2018.1465773. 
  11. ↑ "Home - Anaeromyces robustus v1.0". https://mycocosm.jgi.doe.gov/Anasp1/Anasp1.home.html#:~:text=renewable%20biotechnology%20applications.-,Anaeromyces%20sp.,at%20the%20Santa%20Barbara%20Zoo.. 
  12. ↑ 12.0 12.1 12.2 Schären, M.; Frahm, J.; Kersten, S.; Meyer, U.; Hummel, J.; Breves, G.; Dänicke, S. (2018-05-01). "Interrelations between the rumen microbiota and production, behavioral, rumen fermentation, metabolic, and immunological attributes of dairy cows" (in English). Journal of Dairy Science 101 (5): 4615–4637. doi:10.3168/jds.2017-13736. ISSN 0022-0302. PMID 29454699. https://www.journalofdairyscience.org/article/S0022-0302(18)30141-3/abstract. 
  13. ↑ 13.0 13.1 13.2 13.3 13.4 13.5 Liu, Zhenyang; Wen, Sitao; Wu, Guogan; Wu, Huawei (2022-09-01). "Heterologous expression and characterization of Anaeromyces robustus xylanase and its use in bread making" (in en). European Food Research and Technology 248 (9): 2311–2324. doi:10.1007/s00217-022-04047-2. ISSN 1438-2385. https://doi.org/10.1007/s00217-022-04047-2. 

Wikidata ☰ Q107483491 entry



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