Biology:(S)-2-haloacid dehalogenase

From HandWiki
Short description: Class of enzymes
(S)-2-haloacid dehalogenase
Identifiers
EC number3.8.1.2
CAS number37289-39-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a (S)-2-haloacid dehalogenase (EC 3.8.1.2) is an enzyme that catalyzes the chemical reaction

(S)-2-haloacid + H2O [math]\displaystyle{ \rightleftharpoons }[/math] (R)-2-hydroxyacid + halide

Thus, the two substrates of this enzyme are (S)-2-haloacid and H2O, whereas its two products are (R)-2-hydroxyacid and halide.

This enzyme belongs to the family of hydrolases, specifically those acting on halide bonds in carbon-halide compounds. The systematic name of this enzyme class is (S)-2-haloacid halidohydrolase. Other names in common use include 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase [ambiguous][ambiguous], 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DL-2-haloacid dehalogenase[ambiguous], L-2-haloacid dehalogenase, and L-DEX. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,2-dichloroethane degradation.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1AQ6, 1JUD, 1QH9, 1QQ5, 1QQ6, 1QQ7, 1ZRM, 1ZRN, 2NO4, and 2NO5.

References

  • "Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases". J. Biol. Chem. 243 (2): 428–34. 1968. PMID 5635785. 
  • "Preparation and properties of 2-halo acid dehalogenase from Pseudomonas putida". Agric. Biol. Chem. 46 (3): 837–838. 1982. doi:10.1271/bbb1961.46.837. 
  • "2-Haloacid dehalogenase from a 4-chlorobenzoate-degrading Pseudomonas spec. CBS 3". Hoppe-Seyler's Z. Physiol. Chem. 364 (5): 529–35. 1983. doi:10.1515/bchm2.1983.364.1.529. PMID 6873881. 
  • "Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation". Biochem. Biophys. Res. Commun. 220 (3): 828–33. 1996. doi:10.1006/bbrc.1996.0489. PMID 8607850. 
  • "Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3". Biol. Chem. Hoppe-Seyler 372 (10): 915–22. 1991. doi:10.1515/bchm3.1991.372.2.915. PMID 1772590. 
  • "Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5". Curr. Microbiol. 36 (2): 96–101. 1998. doi:10.1007/s002849900286. PMID 9425247. 
  • "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Arch. Microbiol. 131 (3): 179–83. 1982. doi:10.1007/BF00405875. PMID 7103659. 
  • "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10 (1–3): 57–65. 2000. doi:10.1016/S1381-1177(00)00108-9. 
  • M, Tsunasawa S; Esaki N (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68 (11): 2097–2103. doi:10.1351/pac199668112097.